The best detergent that you can use for this is bleach or clorox. You need 1/2 cup of clorox to 1 gallon of water. You can change the ratio and add more clorox if it makes you feel better.
Spray this on the affected area and allow the sun to dry it. I still do not recommend that you use this area for puppies and other animals without well developed immune systems for at least 6 months.
Let me know if you still have questions.